workshop Summary about flow cytometry

 

workshop Summary

On 11.04.1394, a workshop about flow cytometry and its application was held in the PSRC with supervision of Prof Abdollahi and collaboration of Ms Kazemi from Toba Negin Company. Flow cytometry is routinely used in the diagnosis of health disorders, but has many other applications in basic research, clinical practice and clinical trails. A common variation is to physically sort particles based on their properties, so as to purify populations of interest. The history or background of flow cytometry was first described.  At the 5th American Engineering Foundation Conference on Automated Cytology in Pensacola (Florida) in 1976 - eight years after the introduction of the first fluorescence-based flow cytometer (1968) - it was agreed to use the name "flow cytometry", a term that quickly became popular.

The main components in the modern flow cytometry are as follows:

  • a flow cell - liquid stream (sheath fluid), which carries and aligns the cells so that they pass single file through the light beam for sensing
  • a measuring system which measures the impedance (or conductivity) and optical systems - lamps (mercury, xenon); high-power water-cooled lasers (argon, krypton, dye laser); low-power air-cooled lasers (argon (488 nm), red-HeNe (633 nm), green-HeNe, HeCd (UV)); diode lasers(blue, green, red, violet) resulting in light signals
  • a detector and Analogue-to-Digital Conversion (ADC) system - which generates forward-scattered light (FSC) and side-scattered light (SSC) as well as fluorescence signals from light into electrical signals that can be processed by a computer
  • an amplification system - Linear
  • a computer for analysis of the signals. She also talked how to analyze data with respect to gating and computational analysis through Forward Scattered Channel (FSC) and Side Scattered Channels (SSC). As LASER line beam crosses the single cell line sample and gives output. At the end of theoretical session, a practical session of flow cytometry was started and some blood sampleswere analyzed to detect CD4, CD8, etc. Students enjoyed the workshop.